Notch Reporter Assay Kit (cAMP/PKA pathway)

Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-60509 500 rxns - -

3 - 15 business days*

869.00€
 
Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in... more
Product information "Notch Reporter Assay Kit (cAMP/PKA pathway)"
Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready Notch1 NICD expression vector and CSL (CBF1/RBP-Jkappa) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized CSL responsive element upstream of a minimal promoter. The CSL (CBF1/RBP-Jkappa) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity. Application: Monitor Notch signaling pathway activity, Screen activators or inhibitors of Notch signaling pathway, Study effects of RNAi or gene overexpression on the activity of Notch pathway. Components : CSL (CBF1/RBPJkappa) luciferase reporter vector constitutively expressing Renilla luciferase vector, 500 µl, Negative Control Non-inducible luciferase vector constitutively expressing Renilla luciferase vector, 500 µl, Notch1 NICD Expression vector, 250 µl, Negative Control Expression vector , 250 µl. References: 1. Lu FM et al. (1996). Proc. Natl. Acad. Sci. USA 93(11): 5663-5667. 2. Moellering RE et al. (2009). Nature 462(7270):182-188.
Keywords: Notch reporter, PKA reporter, cAMP reporter, luciferase reporter , CSL reporter, CBF1 reporter, RBP-J reporter,
Supplier: BPS Bioscience
Supplier-Nr: 60509

Properties

Application: Notch activity assays

Database Information

KEGG ID : K02599 | Matching products

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Notch Reporter Assay Kit (cAMP/PKA pathway)"
Write a review
or to review a product.
Viewed