FFAR1 (GPR40) Reporter Assay Kit

FFAR1 (GPR40) Reporter Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay601190-1 1 each -

1 - 10 business days

500.00€
 
Free fatty acid receptor 1 (FFAR1)/GPR40 is a G protein-coupled receptor for long-chain fatty... more
Product information "FFAR1 (GPR40) Reporter Assay Kit"
Free fatty acid receptor 1 (FFAR1)/GPR40 is a G protein-coupled receptor for long-chain fatty acids such as docosahexaenoic acid and eicosapentaenoic acid omega-3 fatty acids. It is involved in the regulation of insulin release from pancreatic beta cells and mediates triglyceride-induced secretion of incretins GLP-1 and GIP from intestinal endocrine cells. Cayman's FFAR1 (GPR40) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for FFAR1, an engineered G protein that directs Galphaq activation signals to the Galphas pathway, and a cAMP response element-regulated secreted alkaline phosphatase (SEAP) reporter (FFAR1 reverse transfection strip plate). Cells grown on the transfection complex will express FFAR1 at the cell surface and the recombinant G protein inside the cell. Binding of agonists to FFAR1 initiates a signal transduction cascade resulting in expression of SEAP, which is secreted into the cell culture medium. Aliquots of culture medium are collected 16-24 hours after stimulation and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is easy to use and can be readily applied to high-throughput screening for therapeutic compounds regulating activation of FFAR1. A known FFAR1 agonist, GW 9508, is included in the kit for use as a positive control. The kit provides sufficient reagent to measure SEAP activity at three time points using the three included white assay plates.
Keywords: Free Fatty Acid Receptor 1, G Protein-Coupled Receptor 40
Supplier: Cayman Chemical
Supplier-Nr: 601190-1

Properties

Application: Cell based assays, HTS, Reverse transfection reporter assay
Reactivity: Human
Format: Solid Phase

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H302, H315, H412
P Phrases: P210, P264, P270, P273, P280, P321, P330, P301+P310, P302+P352, P332+P313, P362+P364, P370+P378, P403, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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