EP2 Receptor (rat) Reporter Assay Kit

EP2 Receptor (rat) Reporter Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay600340-1 100 tests -

1 - 10 business days

500.00€
 
The EP2 receptor is one of four GPCRs that mediate the actions of PGE2. The diverse effects of... more
Product information "EP2 Receptor (rat) Reporter Assay Kit"
The EP2 receptor is one of four GPCRs that mediate the actions of PGE2. The diverse effects of PGE2 acting via EP2 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP2 Receptor (rat) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for rat EP2 receptor and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (EP2 Receptor Reverse Transfection Plate). Cells grown on the transfection complex will express EP2 at the cell surface. Binding of agonists to EP2 initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. Aliquots of media are removed at time intervals, beginning at approximately six hours, and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating the activation of EP2. PGE2 is included in the kit for use as a positive control. The kit provides sufficient reagents to measure SEAP activity at three time points using the black plates included.
Keywords: Ptger2, Prostanoid EP2 receptor, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostaglandin E2 receptor EP2 subtype
Supplier: Cayman Chemical
Supplier-Nr: 600340-1

Properties

Application: Reverse transfection reporter assay
Reactivity: Rat
Format: Homog.

Database Information

KEGG ID : K04259 | Search using KEGG ID
UniProt ID : Q62928 | Search using UniProt ID

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H302, H315, H319, H360
P Phrases: P201, P202, P210, P264, P270, P280, P321, P330, P301+P310, P302+P352, P305+P351+P338, P308+P313, P332+P313, P337+P313, P362+P364, P370+P378, P403, P405, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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