CYP2B6 Induction Reporter Assay Kit

CYP2B6 Induction Reporter Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay600680-100 100 tests -

1 - 10 business days

500.00€
 
Early screening of a drug candidate's potential to induce or inhibit CYP2B6 is a critical step in... more
Product information "CYP2B6 Induction Reporter Assay Kit"
Early screening of a drug candidate's potential to induce or inhibit CYP2B6 is a critical step in the drug development process. Cayman's CYP2B6 Induction Reporter Assay Kit is a novel method to evaluate human Constitutive Androstane Receptor 3 (hCAR3)-mediated CYP2B6 induction for the discovery of hepatic CYP inducers. Our Reverse Transfection Reporter Assays have overcome many of the disadvantages of other transfection approaches. Cayman's CYP2B6 Induction Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing a Secreted Alkaline Phosphatase (SEAP) reporter regulated by the human CYP2B6 gene promoter. The complex also contains two nuclear receptor expression constructs, human Constitutive Androstane Receptor 3 (hCAR3) and human Hepatocyte Nuclear Factor 4alpha (HNF4alpha). Cells grown on the CYP2B6 Reverse Transfection Strip Plate will introduce the reporter gene and express CAR3 and HNF4alpha. Binding of inducer-activated receptor and transcription factors to the CYP2B6 promoter initiates a signal resulting in expression of SEAP, which is secreted into the cell culture medium. Aliquots of medium are removed at time intervals beginning at about 24 hours and SEAP activity is measured simply by adding a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high-throughput screening for potential CAR-mediated CYP2B6 inducers. A known CYP2B6 inducer, CITCO, is included in the kit for use as a positive control.
Keywords: CYP2B6 Induction STEP Reporter Assay Kit (Luminescence)
Supplier: Cayman Chemical
Supplier-Nr: 600680-1

Properties

Application: Reverse transfection reporter assay
Format: Solid Phase

Database Information

KEGG ID : K17709 | Search using KEGG ID

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Warning
H Phrases: H412
P Phrases: P210, P273, P280, P332+P313, P370+P378, P403, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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