LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay601170-96 96 wells -

approx. 1-3 business days

83.00€
Cay601170-480 480 wells -

1 - 10 business days

310.00€
 
Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability,... more
Product information "LDH Cytotoxicity Assay Kit"
Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, while apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from cell- or compound-induced cytotoxicity. LDH activity, therefore, can be used as an indicator of cell membrane integrity and serve as a general means to assess cell viability by measuring plasma membrane permeability. Cayman's LDH Cytotoxicity Assay Kit measures cell death in response to chemical compounds or environmental factors using a coupled two-step reaction. In the first step, LDH catalyzes the reduction of NAD+ to NADH and H+ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H+ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan which absorbs strongly at 490-520 nm.
Keywords: Lactate Dehydrogenase Cytotoxicity Assay Kite, raplacement of Cay10008882
Supplier: Cayman Chemical
Supplier-Nr: 601170-96

Properties

Application: Cell based assays
Reactivity: Broad
Format: Homog.

Database Information

KEGG ID : K00016 | Search using KEGG ID

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H319
P Phrases: P264, P280, P305+P351+P338, P337+P313
Caution
Our products are for laboratory research use only: Not for administration to humans!
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