Bucculite(TM) XdU Cell Proliferation Fluorescence Imaging Kit *Deep Red Fluorescence*

Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Bucculite(TM) XdU Cell Proliferation Fluorescence Imaging Kit uses XdU which is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated XdU is labelled with iFluor(TM) 647 azide. The resulted iFluor(TM) 647-labeled DNA formed in cells is imaged with a Cy5 filter set. Bucculite(TM) XdU Cell Proliferation Fluorescence Imaging Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry-based assay. It is sensitive and might be used for measuring active DNA synthesis at single-cell level.