Cell Meter(TM) Cell Adhesion Assay Kit

The Cell Meter(TM) Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with Calcein UltraGreen AM and allowed to adhere. After removal of nonadherent cells, The fluorescence of Calcein UltraGreen is used to calculate the number of adherent cells. The use of our outstanding fluorogenic dye, Calcein UltraGreen AM provides a fast and sensitive method for measuring cell adhesion with a variety of cell types. Calcein UltraGreen AM is nonfluorescent but, once loaded into cells, is cleaved by endogenous esterases to produce highly fluorescent Calcein UltraGreen, a brightly fluorescent, pH-independent, cytoplasmic cell marker with the minimal interference to cell adhesion process. The Cell Meter(TM) cell adhesion assay is designed for use with fluorescence microplate readers. The robust performance of Calcein UltraGreen AM and simple procedure of the kit avoids problems associated with assays that utilize radioisotopes, which generate hazardous waste, and with assays that rely on the use of covalently coupled cell-surface labels, which can potentially alter cell function.