Screen Quest(TM) Fluo-4 No Wash Calcium Assay Kit

Screen Quest(TM) Fluo-4 No Wash Calcium Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-36325 10 plates -

2 - 8 business days

427.00€
ABD-36326 100 plates -

2 - 8 business days

3,186.00€
 
Ex: 490 nm. Em: 525 nm. Calcium flux assays are preferred methods in drug discovery for screening... more
Product information "Screen Quest(TM) Fluo-4 No Wash Calcium Assay Kit"
Ex: 490 nm. Em: 525 nm. Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen QuestT Fluo-4 No Wash Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fluo-4 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-4 AM are cleaved by non-specific cell esterase, resulting in a negatively charged Fluo-4 dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-4. This Screen Quest Fluo-4 No Wash Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
Supplier: AAT Bioquest
Supplier-Nr: 36325

Properties

Format: Desiccated

Database Information

Handling & Safety

Storage: -20°C (protect from light)
Shipping: +20°C (International: +20°C)
Signal Word: Warning
P Phrases: P304, P312
Caution
Our products are for laboratory research use only: Not for administration to humans!
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