TUNEL in situ Apoptosis Kit (HRP-DAB method)

TUNEL in situ Apoptosis Kit (HRP-DAB method)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
E-CK-A331.20 20 tests -

7 - 16 business days*

277.00€
E-CK-A331.50 50 tests -

7 - 16 business days*

475.00€
E-CK-A331.100 100 tests -

7 - 16 business days*

772.00€
 
Elabscience(R) TUNEL Apoptosis Assay Kit is a highly sensitive, rapid and simple method for cell... more
Product information "TUNEL in situ Apoptosis Kit (HRP-DAB method)"
Elabscience(R) TUNEL Apoptosis Assay Kit is a highly sensitive, rapid and simple method for cell apoptosis detection. After Biotin-dUTP labeling and DAB staining, cell apoptosis can be detected under ordinary optical microscopy. This kit is developed to detect tissue (Paraffin embedding, freezing and ultrathin section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection. Detection principle: When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.
Supplier: Elabscience
Supplier-Nr: E-CK-A331

Properties

Application: TUNEL assay, In situ apoptosis detection, Histochemistry
Species reactivity: broad

Database Information

Handling & Safety

Storage: vT (protect from light)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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