Caspase 9, Apoptosis Detection, Fluorometric BioAssay(TM) Kit (ICE-LAP6, Mch6, Apaf 3)

Caspase 9, Apoptosis Detection, Fluorometric BioAssay(TM) Kit (ICE-LAP6, Mch6, Apaf 3)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C2088-05.25 25 tests - -

1 - 19 business days

428.00€
C2088-05.100 100 tests - -

1 - 19 business days

647.00€
C2088-05.200 200 tests - -

1 - 19 business days

818.00€
 
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9... more
Product information "Caspase 9, Apoptosis Detection, Fluorometric BioAssay(TM) Kit (ICE-LAP6, Mch6, Apaf 3)"
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (lambdamax 400nm), upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (lambdamax 505nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Components: 25 assays 100 assays Cell Lysis Buffer 25ml 100ml, 2X Reaction Buffer 2ml 4 x 2ml, LEHD-AFC Substrate (1mM) 125ul 500ul, DTT (1M) 100ul 400ul, Stability: Store kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions.,
Supplier: United States Biological
Supplier-Nr: C2088-05

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Storage: -20°C
Shipping: +4°C (International: °C)
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Our products are for laboratory research use only: Not for administration to humans!
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