Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. USBio's Caspase-8 Apoptosis Detection Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity. , Kit Contents:, Assays 25 100 200 400, Cell Lysis Buffer 25ml 100ml 100ml 100ml, 2X Reaction Buffer 2ml 4x2ml 16ml 32ml , IETD-AFC (1mM) 0.125ml 0.5ml 2x.05ml 2x1ml, DT (1M) 0.1ml 0.4ml 0.4ml 0.4ml, , Storage and Stability: Store kit at -20°C before use. After opening, store Cell Lysis Buffer and 2X Reaction Buffer at 4°C. All reagents are stable for 6 months under proper storage conditions.