Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssay(TM) Kit

Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssay(TM) Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C2086-74.25 25 tests - -

1 - 19 business days

433.00€
C2086-74.200 200 tests - -

1 - 19 business days

818.00€
 
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The... more
Product information "Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssay(TM) Kit"
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2/ICH-1 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity. Components: 25 assays 100 assays Cell Lysis Buffer 25ml 100ml, 2X Reaction Buffer 2ml 4 x 2ml, VDVAD-pNA Substrate (4mM) 125ul 500ul, DTT (1M) 100ul 400ul, Dilution Buffer 25ml 100ml, Stability: Store kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions., ,
Supplier: United States Biological
Supplier-Nr: C2086-74

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Storage: -20°C
Shipping: +4°C (International: °C)
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Our products are for laboratory research use only: Not for administration to humans!
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