Calpain, Fluorometric BioAssay(TM) Kit

Calpain, Fluorometric BioAssay(TM) Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C1035-31B.100 100 tests - -

1 - 19 business days

Activation of calpain is involved in many forms of physiological and pathological processes... more
Product information "Calpain, Fluorometric BioAssay(TM) Kit"
Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca2, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity. The Extraction Buffer provided with the kit specifically extracts cytosolic proteins without contaminations of cell membrane and lysosome proteases. The Extraction Buffer also prevents auto-activation of calpain during the extraction procedure. Thus, the kit detects only activated calpain in cytosol upon treatment of cells with inducers (e.g., chemicals or drugs). The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (lmax=400nM), upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (lmax=505nM), which can be quantified using a fluorometer or a fluorecence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity. Intended Use: The kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection of the cleavage of calpain substrate at 505nm using a fluorometer or a fluorecence plate reader. Sample Type: Cell and tissue lysates, Specificity: Mammalian, Absorption: 400nm , Excitement: 505nm, Fluorescence: Ex/Em 400/505nm , Kit Components: , *C1035-31B1: Extraction Buffer, 1x25ml, *C1035-31B2: Reaction Buffer 10X, 1x1.5ml, C1035-31B3: Calpain Substrate Ac-LLY-AFC, 1x500ul , C1035-31B4: Active Calpain I (Positive Control), 1x10ul, C1035-31B5: Calpain Inhibitor Z-LLY-FMK, 1x10ul, Storage and Stability: Store components at -70°C. Once *C1035-31B1 and *C1035-31B2 are opened, store at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: C1035-31B


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Storage: -80°C
Shipping: -20°C (International: °C)
Our products are for laboratory research use only: Not for administration to humans!
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