Cell-Based Assays

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Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence)
Secreted Alkaline Phosphatase Reporter Gene...

Item number: Cay600260-300

Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman's Secreted Alkaline Phosphatase Reporter Gene Assay Kit...
Application: Chemiluminescence
Format: Homog.
287.00€
Review
Annexin V FITC Assay Kit
Annexin V FITC Assay Kit

Item number: Cay600300-100

One of the hallmarks of the early stages of apoptosis is the redistribution of membrane phospholipids such as phosphatidylserine and phosphatidylethanolamine from the inner to outer leaflet of the membrane bilayer where they are exposed on the cell surface. Externalization of phosphatidylserine residues to the outer plasma membrane leaflet allows their detection via their...
Application: Cell based assays, FC, Fluorescence
Format: Homog.
350.00€
Review
Sulfenylated Protein Cell-Based Detection Kit
Sulfenylated Protein Cell-Based Detection Kit

Item number: Cay600320-96

Reactive oxygen species (ROS) react with proteins, resulting in protein modification, such as protein sulfenylation through the reversible oxidation of cysteine residues. Redox-sensitive cysteine residues in proteins can function as sensors of ROS and serve as molecular switches, activating or deactivating proteins, following a change in oxidative state. However, the...
Application: FC, Fluorescence
407.00€
Review
EP2 Receptor (rat) Reporter Assay Kit
EP2 Receptor (rat) Reporter Assay Kit

Item number: Cay600340-1

The EP2 receptor is one of four GPCRs that mediate the actions of PGE2. The diverse effects of PGE2 acting via EP2 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP2 Receptor (rat) Reporter Assay Kit consists of a 96-well plate...
Application: Reverse transfection reporter assay
Reactivity: Rat
Format: Homog.
500.00€
Review
EP4 Receptor (rat) Reporter Assay Kit
EP4 Receptor (rat) Reporter Assay Kit

Item number: Cay600350-100

The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP4 Receptor (rat) Reporter Assay Kit consists...
Application: Reverse transfection reporter assay
Reactivity: Rat
Format: Homog.
500.00€
Review
Glutathione Cell-Based Detection Kit (Blue Fluorescence)
Glutathione Cell-Based Detection Kit (Blue...

Item number: Cay600360-96

Glutathione (GSH) is the most prevalent low molecular-weight tripeptide thiol in animal cells. It serves as a primary cellular anti-oxidant and plays a fundamental role in the elimination of environmental toxins. During early apoptosis, cells may exclude GSH, causing a decrease in intracellular GSH levels. The intracellular level of GSH is thus used as an indicator of cell...
Application: Fluorescence
Format: Homog.
332.00€
Review
Multi-Drug Resistance Assay Kit (Calcein AM)
Multi-Drug Resistance Assay Kit (Calcein AM)

Item number: Cay600370-1000

P-Glycoprotein (P-gp), the product of the multidrug resistance (MDR) gene MDR1, and multidrug resistance associated proteins (MRP) are members of the superfamiliy of ATP-binding cassette (ABC) transporters. They are localized in the luminal membrane of endothelial cells and function to detoxify or eliminate a spectrum of harmful agents and chemicals by mediating...
Application: MDR protein modulator studies
Format: Homog.
282.00€
Review
EP4 Receptor (rat) Activation Assay Kit (cAMP)
EP4 Receptor (rat) Activation Assay Kit (cAMP)

Item number: Cay600410-1

The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP4 Receptor (rat) Activation Assay Kit (cAMP)...
Application: Reverse transfection reporter assay
Reactivity: Rat
Format: Solid Phase
563.00€
Review
Cholesterol Uptake Cell-Based Assay Kit
Cholesterol Uptake Cell-Based Assay Kit

Item number: Cay600440-1

Maintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. However, the mechanisms controlling cholesterol absorption are not well understood. A better understanding of the relationship between cholesterol...
Application: FC
Format: Homog.
232.00€
Review
Glycolysis Cell-Based Assay Kit
Glycolysis Cell-Based Assay Kit

Item number: Cay600450-1

Cayman's Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between...
Application: Extracellular L-lactate detection
Format: Homog.
313.00€
Review
NAD/NADH Cell-Based Assay Kit
NAD/NADH Cell-Based Assay Kit

Item number: Cay600480-1

Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman's NAD/NADH Cell-Based Assay Kit provides a...
Application: Measure intracellular NAD?
Format: Homog.
369.00€
Review
Phagocytosis Assay Kit (IgG PE)
Phagocytosis Assay Kit (IgG PE)

Item number: Cay600540-1

Cayman's Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the...
Application: FC
Format: Bead Based
344.00€
Review
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