Cell-based Assays

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of cell viability, cytotoxicity and biological activity. By realistically representing the situation in vivo, these assays enable direct analysis of cell responses and play an important role in understanding disease mechanisms.

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of... read more »
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Cell-based Assays

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of cell viability, cytotoxicity and biological activity. By realistically representing the situation in vivo, these assays enable direct analysis of cell responses and play an important role in understanding disease mechanisms.

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Cell Navigator(TM) Lysosome Staining Kit *NIR Fluorescence*
Cell Navigator(TM) Lysosome Staining Kit *NIR Fluorescence*

Item number: ABD-22652

The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). Our Cell Navigator(TM) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as...
Application: Fluorescence imaging
279.00€ *
Review
Protein Synthesis Assay Kit
Protein Synthesis Assay Kit

Item number: Cay601100-96

Cayman's Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating polypeptide chains, thereby...
Application: FC, fluorescence
543.00€ *
Review
AR (human) Reporter Assay Kit
AR (human) Reporter Assay Kit

Item number: IB-IB03001

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO's reporter cells include the luciferase...
Keywords: AR, DHTR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Application: Functional activity screening
Species reactivity: human
From 1,448.00€ *
Review
One-Step Luciferase Assay System
One-Step Luciferase Assay System

Item number: BPS-60690-1

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component...
Keywords: Luciferin, Firefly luciferase, luciferase buffer,
Application: Firefly luciferase assays, HTS
From 238.00€ *
Review
Cell Meter(TM) TUNEL Apoptosis Assay Kit
Cell Meter(TM) TUNEL Apoptosis Assay Kit

Item number: ABD-22844

DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that...
Application: TUNEL apoptosis assay
418.00€ *
Review
Oxygen Consumption Rate Assay Kit
Oxygen Consumption Rate Assay Kit

Item number: Cay600800-96

The oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells. Oxygen consumption is...
Keywords: OCR
Application: Fluorescence
542.00€ *
Review
FLICA(TM) 660 Caspase 3/7 Assay Kit, far-red fluorescence  (25-50 tests)
FLICA(TM) 660 Caspase 3/7 Assay Kit, far-red fluorescence...

Item number: ICT-9125

The far-red FLICA® 660-DEVD-FMK caspase-3 detection probe is comprised of the preferred affinity peptide sequence (DEVD) targeted by activated caspase-3 and caspase-7, a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting fluorescent caspase-3 inhibitor probe forms an...
Keywords: Apoptosis kit, CASP3, CPP32, SCA-1, CPP-32, CASP-3, Apopain, Caspase-3, EC=3.4.22.56, Protein Yama, Cysteine protease...
Application: FM, FC, Apoptosis detection
367.00€ *
Review
FAM-FLICA(R) Caspases 3 & 7 Assay Kit
FAM-FLICA(R) Caspases 3 & 7 Assay Kit

Item number: ICT-93

This kits makes use of a cell-permeant green fluorecent probe (FAM-DEVD-FMK ) that binds to active caspase-3 & 7 in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.
Keywords: Apoptosis kit
Application: FM, FC, Apoptosis detection
310.00€ *
Review
Cell Meter(TM) JC-10 Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays
Cell Meter(TM) JC-10 Mitochondrial Membrane Potential...

Item number: ABD-22801

Although JC-1 is widely used in many labs, its poor water solubility causes extraordinary inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, JC-10 has much better...
Application: FC, Apoptosis studies
418.00€ *
Review
ADRA1B Reporter Assay System (human)
ADRA1B Reporter Assay System (human)

Item number: IB-IB31101

This is an all-inclusive cell-based luciferase reporter assay kit targeting the the Human Adrenoreceptor Alpha 1B (ADRA1B). INDIGO's ADRA1A reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ADRA1B. In addition to ADRA1B Reporter Cells, this kit...
Keywords: ADRA1B, Alpha-1B adrenoceptor, Alpha-1B adrenoreceptor, Alpha-1B adrenergic receptor
Application: Functional activity screening
Species reactivity: human
1,448.00€ *
Review
ADRA1B Reporter Assay System (human)
ADRA1B Reporter Assay System (human)

Item number: IB-IB31102

This is an all-inclusive cell-based luciferase reporter assay kit targeting the the Human Adrenoreceptor Alpha 1B (ADRA1B). INDIGO's ADRA1A reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ADRA1B. In addition to ADRA1B Reporter Cells, this kit...
Keywords: ADRA1B, Alpha-1B adrenoceptor, Alpha-1B adrenoreceptor, Alpha-1B adrenergic receptor
Application: Functional activity screening
Species reactivity: human
3,803.00€ *
Review
ADRA1D Reporter Assay System (human)
ADRA1D Reporter Assay System (human)

Item number: IB-IB31201

This is an all-inclusive cell-based luciferase reporter assay kit targeting the the Human Adrenoreceptor Alpha 1D (ADRA1D). INDIGO's ADRA1A reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ADRA1D. In addition to ADRA1D Reporter Cells, this kit...
Keywords: ADRA1A, ADRA1D, Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, Alpha-1A adrenergic receptor, Alpha-adrenergic receptor...
Application: Functional activity screening
Species reactivity: human
1,448.00€ *
Review
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