The PARP2 Homogeneous Assay Kit comes in a convenient AlphaLISA(R) format, with biotinylated histone substrate, activated DNA, primary antibody, PARP assay buffer, and purified PARP2 for 384 enzyme reactions. The key to the PARP2 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, a sample containing PARP2 enzyme is incubated with activated DNA and biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.