• Please note that we do not deliver goods between 19.12.2019 and 03.01.2020 due to public holidays. However, we are happy to accept orders for deliveries in 2020 during this period.

PAF Acetylhydrolase Assay Kit

PAF Acetylhydrolase Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay760901-96 96 wells -

1 - 10 business days

262.00€
 
Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety... more
Product information "PAF Acetylhydrolase Assay Kit"
Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins. The Cayman Chemical PAF acetylhydrolase assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB, Ellman's reagent). The dynamic range of the assay is only limited by the accuracy of the absorbance measurement. Most plate readers are linear to an absorbance of 1.2. The detection range of the assay is from 0.02 to 0.2 µmol/min/ml of PAF-AH activity which is equivalent to an absorbance increase of 0.01 to 0.1 per minute. Each kit contains assay buffer, DTNB, 2-thio PAF (substrate), human PAF-AH standard, a 96 well plate, and complete instructions.
Keywords: PAFAH, PLA2G7, gVIIA-PLA2, LDL-PLA(2), EC=3.1.1.47, PAF 2-acylhydrolase, PAF acetylhydrolase, Group-VIIA phospholipase A2, LDL-associated phospholipase A2, Platelet-activating factor acetylhydrolase, 2-acetyl-1-alkylglycerophosphocholine esterase
Supplier: Cayman Chemical
Supplier-Nr: 760901-96

Properties

Application: PAF Acetylhydrolase activity detection
Reactivity: Human
Format: Homog.

Database Information

KEGG ID : K01062 | Search using KEGG ID
UniProt ID : Q13093 | Search using UniProt ID

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H225, H315, H319, H335
P Phrases: P210, P240, P241, P242, P243, P261, P264, P271, P280, P312, P321, P302+P352, P303+P361+P353, P304+P340, P305+P351+P338, P332+P313, P337+P313, P362+P364, P370+P378, P405, P403+P233, P403+P235, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "PAF Acetylhydrolase Assay Kit"
Write a review
or to review a product.
Viewed