IL-1beta (Human) Colorimetric ELISA Detection Kit

The cytokine protein Interleukin-1beta is a mediator in the inflammatory response and is a therapeutic target for autoinflammatory diseases such as Alzheimer's disease and Schnitzler syndrome. The Colorimetric Human IL-1beta Detection Kit is designed for detecting and quantifying human interleukin-1beta in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-1beta are incubated on the coated plate followed by detecting the captured IL-1 beta with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.