GLP SAM-Screener(TM) Assay Kit

GLP SAM-Screener(TM) Assay Kit

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Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay600570-384 384 wells -

1 - 10 business days

Cay600570-1920 1920 wells -

1 - 10 business days

Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes... more
Product information "GLP SAM-Screener(TM) Assay Kit"
Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of the epsilon amino group of lysine. G9a-like protein (GLP) is a SET domain-containing methyltransferase that specifically mono- and di-methylates histone H3 at lysine 9 (H3K9). GLP and G9a function as major euchromatic H3K9me1 and H3K9me2 histone methyltransferases and also have been found to methylate several nonhistone substrates, including p53(K372). This fluorescence polarization assay is based upon a proprietary small molecule fluorescent probe* that binds to the SAM binding pocket in GLP. Binding of the small molecule probe to GLP induces an increase in fluorescence polarization. Binding of the probe can be competed with the endogenous cofactor SAM or by the inhibitor sinefungin, but is unaffected by the histone H3 peptide substrate. The GLP SAM-Screener(TM) Assay is robust (Z' >0.5) and exhibits a greater than 100 mP shift over a range of 0-250 nM GLP. The assay is suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well) if desired. *United States Patent 9,120,820
Keywords: GLP, GLP1, EHMT1, EUHMTASE1, Eu-HMTase1, EC=2.1.1.-, EC=, H3-K9-HMTase 5, G9a-like protein 1, Lysine N-methyltransferase 1D, Histone H3-K9 methyltransferase 5, Histone-lysine N-methyltransferase EHMT1
Supplier: Cayman Chemical
Supplier-Nr: 600570-384


Application: Fluorescence, HTS
Format: Homog.

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Shipping: -20°C (International: -80°C)
Our products are for laboratory research use only: Not for administration to humans!
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