Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit

Item number Size Datasheet Manual SDS Delivery time Quantity Price
SBB-KF0120-KI01 1 kit - -

3 - 9 business days*

1,433.00€
 
South Bay Bio's Cullin4a/Rbx1/DDB1/CRBN Neddylated E3 Ligase TR-FRET Kit provides a fast and... more
Product information "Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit"
South Bay Bio's Cullin4a/Rbx1/DDB1/CRBN Neddylated E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto the E3 complex in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto CRBN (or substrates) in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate "true" signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
Keywords: CUL4A, CUL-4A, Cullin-4A
Supplier: South Bay Bio
Supplier-Nr: KF0120

Properties

Application: Compound Screening
Species reactivity: human
Format: Homog.

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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