Cell Navigator(TM) Fluorimetric Lipid Droplet Assay Kit *Red Fluorescence*

Ex: 550 nm. Em: 640 nm. Lipid droplets, also referred to as lipid bodies, oil bodies or adiposomes, are lipid-rich cellular organelles that regulate the storage and hydrolysis of neutral lipids. They also serve as a reservoir of lipid source for many important biological processes such as fatty acid and cellular cholesterol for energy and membrane formation and maintenance. Abnormal accumulation of the cytoplasmic lipid droplets occurs in a variety of pathological conditions and can be an indicator of metabolic deficiency or pathogenesis. AAT Bioquest's Cell NavigatorT Fluorimetric Lipid Droplet Assay Kit is a robust tool that could quantitatively measure lipid droplet accumulation. DropliteT Red is used in the kit for lipophilic stain. DropliteT Red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets with fluorescence microscopy, flow cytometry or fluorescence microplate reader. The red fluorescence signal could be read at Ex/Em=550/640 nm or observed using the filter set of TRITC.