ATP Colorimetric/Fluorometric BioAssayKit

Item number Size Datasheet Manual SDS Delivery time Quantity Price
A0015.100 100 tests -

3 - 19 business days*

787.00€
 
ATP is the primary energy currency of living systems. Virtually all energy requiring processes... more
Product information "ATP Colorimetric/Fluorometric BioAssayKit"
ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. ATP Colorimetric and Fluorometric BioAssay(TM) kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (Lmax = 570nm) or fluorometric (Ex/Em = 535/587nm) methods. The assay can detect as low as 50picomol (1uM) of ATP in various samples. The kit provides sufficient reagents for 100 assays. Kit Components: A0015-01: ATP Assay Buffer, 1x25ml A0015-02: ATP Probe (in DMSO), 1x200ul, A0015-04: ATP Converter (lyophilized), 1x1 vial, A0015-05: Developer Mix (lyophilized), 1x1 vial , A0015-06: ATP Standard (1umol, lyophilized), 1x1 vial , Storage and Stability: Store at -20°C. Aliquots are stable for 2 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: A0015

Properties

Application: ATP detection assays
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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