Amplite(TM) Luminometric Nitroreductase Assay Kit

Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds. They are absent in mammalian cells but widespread in bacteria. Nitroreductases play a crucial role in the reduction of nitroaromatic compounds via NAD(P)H-dependent reactions. NTR are also targets for developing novel antibiotics, degradation of pollutants, and cancer therapy. Although the quantification of NTR becomes extremely important for a number of biological applications, most of the current NTR assays are based on ELISA format with low sensitivity and low throughput. Amplite(TM) Luminometric Nitroreductase Assay Kit provides a highly sensitive and convenient method to quantify NTR activity. The kit uses a luciferin derivative that can be selectively reduced by NTR luciferin. The amount of generated luciferin is conveniently detected with the well-known luciferase detection system. The luminescent intensity generated is proportional to the NTR activity. This luminometric nitroreductase assay kit has been formulated to have minimal hands-on time and stable luminescence signal. It can detect as low as 20 ng/mL of NTR.