Amplite(TM) Human Serum Albumin (HSA) Site II Binding Assay Kit

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner. Several different ligand binding sites have been identified for HSA. Among them, Site II has been identified as one of major drug binding sites. Amplite(TM) Human Serum Albumin (HSA) Site II Binding Assay Kit is a fluorescence-based high throughput assay to determine the small molecule binding towards HSA. This assay is based on a novel fluorescent probe, HSA Blue(TM) S2. It has been characterized to bind to the site 2 of HSA with unique spectroscopic and binding properties. HSA Blue(TM) S2 displays a large fluorescence intensity difference between the protein-bound and protein-unbound state. The competition of small molecules for HSA binding in the presence of HSA Blue(TM) S2 results in low fluorescence intensities. This assay can be used as a high throughput screen tool to determine total binding to HSA at Site II.