Amplite(TM) Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*

Amplite(TM) Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-12603 1 kit (200 assays) -

3 - 8 business days*

346.00€
 
Ex: 571 nm. Em: 585 nm. E. coli _-galactosidase is a 464 kD tetramer. Each unit of... more
Product information "Amplite(TM) Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*"
Ex: 571 nm. Em: 585 nm. E. coli _-galactosidase is a 464 kD tetramer. Each unit of _-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, _-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of _-galactosidase per cell. This kit uses a red fluorogenic galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The non-fluorescent substate generates a strongly fluorescent product upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. Amplite(TM) Fluorimetric Beta-Galactosidase Assay Kit comes with all the essential components with an optimized assay protocol. It can be used with a fluorescence microplate reader, a fluorescence microscope, or a flow cytometer. It might also be used for screening galactosidase inhibitors or inducers.
Supplier: AAT Bioquest
Supplier-Nr: 12603

Properties

Application: IF, FC, FC
Format: Desiccated

Database Information

Handling & Safety

Storage: -20°C (protect from light)
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow. more
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Amplite(TM) Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*"
Write a review
or to review a product.
Viewed