Amplite(TM) Fluorimetric beta-Galactosidase Assay Kit *Green Fluorescence*

Amplite(TM) Fluorimetric beta-Galactosidase Assay Kit *Green Fluorescence*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-12601 1 kit (500 assays) -

3 - 8 business days*

346.00€
 
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five... more
Product information "Amplite(TM) Fluorimetric beta-Galactosidase Assay Kit *Green Fluorescence*"
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies in this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell. This kit uses a fluorogenic galactosidase substrate that can sensitively distinguish LacZ vs. LacZ-cells. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. The galactosidase-cleaved product has Ex/Em = 490/520 nm that can be detected with most of fluorescence instruments equipped with a FITC filter set.
Keywords: EC=3.2.1.23
Supplier: AAT Bioquest
Supplier-Nr: 12601

Properties

Application: Galactosidase assays
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
Signal Word: Warning
Caution
Our products are for laboratory research use only: Not for administration to humans!
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