Amplite(TM)Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

Amplite(TM)Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-11953 1 kit (500 assays) -

3 - 8 business days*

230.00€
 
Alkaline phosphatase (ALP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate... more
Product information "Amplite(TM)Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*"
Alkaline phosphatase (ALP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. An important use of alkaline phosphatase is as a label for enzyme immunoassays. Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical as well as Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Our Amplite(TM) Fluorimetric Alkaline Phosphatase Assay Kit uses our FDP, a fluorogenic phosphatase substrate, to quantify the alkaline phosphatase activity in solutions, in cell extracts, and on solid surfaces (such as PVDF membranes). It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/525 nm. The kit provides an optimized 'mix and read' assay protocol which is compatible with HTS liquid handling instruments.
Keywords: AP, ALP, EC=3.1.3.1
Supplier: AAT Bioquest
Supplier-Nr: 11953

Properties

Application: Alkaline phosphatase quantification, HTS
Format: Homog.

Database Information

KEGG ID : K01077 | Find alternatives

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Signal Word: Warning
Caution
Our products are for laboratory research use only: Not for administration to humans!
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