Amplite(TM) Colorimetric Total NADP and NADPH Assay Kit *Enhanced Sensitivity*

Amplite(TM) Colorimetric Total NADP and NADPH Assay Kit *Enhanced Sensitivity*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-15276 400 tests -

3 - 8 business days*

346.00€
 
Ex: 635 nm. Soluble in: DMSO. Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine... more
Product information "Amplite(TM) Colorimetric Total NADP and NADPH Assay Kit *Enhanced Sensitivity*"
Ex: 635 nm. Soluble in: DMSO. Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This AmpliteT NADP/NADPH Assay Kit provides a convenient method for sensitive detection of NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction. There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Compared to Kit #15260, this kit has higher sensitivity.
Supplier: AAT Bioquest
Supplier-Nr: 15276

Properties

Application: Cell metabolism assay
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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