Acetyl-CoA BioAssayKit

Item number Size Datasheet Manual SDS Delivery time Quantity Price
A0225-02.100 100 tests - -

3 - 19 business days*

745.00€
 
Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and... more
Product information "Acetyl-CoA BioAssayKit"
Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and breakdown of larger molecules. Acetyl CoA is key in synthetic pathways leading to sesquiterpenes, precursors to cholesterol and other sterols, flavenoids and other polyketides, polyenes and long-chain fatty acids. It is the source of the acetyl group used in histone acetylation. The acetyl group is also incorporated into a variety of other molecules such as acetylcholine, melatonin, heme and TCA cycle intermediates. Acetyl-CoA BioAssay(TM) Kit is a highly sensitive assay for determining Acetyl CoA levels in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe(R) to generate fluorescence (Ex/Em=535/587nm). , The assay can detect 10 to 1000pmol of Acetyl CoA (with detection limit ~0.4uM) in a variety of samples. Kit Components: A0225-02A: Acetyl CoA Assay Buffer, 1x25ml, A0225-02B: PicoProbe(R), 1x200ul , A0225-02C: Conversion Enzyme, 1x100ul , A0225-02D: Acetyl CoA Enzyme Mix, 1x500ul , A0225-02E: Acetyl CoA Substrate Mix, 1x220ul , A0225-02F: CoA Quencher, 1x1ml, A0225-02G: Quench Remover, 1x220ul, A0225-02H: Acetyl CoA Standard (1umol), 1x1 vial, Storage and Stability: Store kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature , before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: A0225-02

Properties

Application: Acetyl-CoA quantification
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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