Biochemical Assays

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NETosis Assay Kit
NETosis Assay Kit

Item number: Cay601010-1

Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore...
Application: NET generation assay
Format: Misc.
388.00€
Review
Oct4 Transcription Factor Assay Kit
Oct4 Transcription Factor Assay Kit

Item number: Cay601080-96

Octamer-binding transcription factor 4 (Oct4) is an essential regulator of embryonic stem cell pluripotency and used as a marker for undifferentiated cells. It also plays a role in gene expression, DNA repair and/or replication, signal transduction and tumorigenesis. Cayman's Oct4 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific...
Application: Oct4 DNA binding assay
Reactivity: Mouse
Format: Solid Phase
532.00€
Review
Neutrophil/Monocyte Respiratory Burst Assay Kit
Neutrophil/Monocyte Respiratory Burst Assay Kit

Item number: Cay601130-1

Respiratory burst, or the rapid generation of reactive oxygen species from immune cells, is crucial for the destruction of invading microorganisms by phagocytes. During this process, professional phagocytes convert molecular oxygen to superoxide anions through the action of NADPH oxidase, which is then converted to reactive oxygen species with potent antimicrobial activity....
Application: FC
Reactivity: Broad
Format: Homog.
369.00€
Review
Multi-Parameter Apoptosis Assay Kit
Multi-Parameter Apoptosis Assay Kit

Item number: Cay601280-1

Cayman's Multi-Parameter Apoptosis Assay Kit is a simple way to probe for multiple different apoptotic readouts at the same time. This kit employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells, TMRE as a probe for Deltapsim, RedDotTM2 as an indicator of plasma membrane permeability/cell viability, and Hoechst Dye to...
Application: Cell based assays, FC, Fluorescence, Multiplex
Format: Homog.
438.00€
Review
EP2 Receptor (human) Reporter Assay Kit
EP2 Receptor (human) Reporter Assay Kit

Item number: Cay601380-96

Prostaglandin E2 (PGE2) is one of the most important biologically active prostanoids which exerts its actions mainly by binding to four distinct E-type prostanoid receptors: EP1, EP2, EP3, and EP4. These four G protein-coupled receptors (GPCRs) exhibit differences in signal transduction mechanisms, tissue localization, and regulation of expression. EP2 receptors are...
Application: Cell based assays, Chemiluminescence, HTS, Reverse transfection reporter assay
Format: Solid Phase
500.00€
Review
EP4 Receptor (human) Reporter Assay Kit
EP4 Receptor (human) Reporter Assay Kit

Item number: Cay601390-96

Prostaglandin E2 (PGE2) is one of the most important biologically active prostanoids which exerts its actions mainly by binding to four distinct E-type prostanoid receptors: EP1, EP2, EP3, and EP4. These four G protein-coupled receptors (GPCRs) exhibit differences in signal transduction mechanisms, tissue localization, and regulation of expression. EP4 receptors are highly...
Application: Cell based assays, Chemiluminescence, HTS, Reverse transfection reporter assay
Format: Solid Phase
500.00€
Review
Mitochondrial PT Pore Assay Kit
Mitochondrial PT Pore Assay Kit

Item number: Cay601430-100

Cayman's Mitochondrial PT Pore Assay Kit utilizes the calcein/cobalt technique multiplexed with TMRE, allowing for the measurement of both membrane integrity and mitochondrial membrane potential (Deltapsim). This assay has been adapted for use with flow cytometry and fluorescence microscopy, and includes controls for Deltapsim depolarization (FCCP) and calcein quenching...
Application: Cell based assays, FC, Fluorescence
Format: Homog.
525.00€
Review
LDL Uptake Flow Cytometry Assay Kit
LDL Uptake Flow Cytometry Assay Kit

Item number: Cay601470-48

Cayman's LDL Uptake Flow Cytometry Assay Kit employs human LDL conjugated to DyLight(TM) 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow cytometry provides the advantage of assessing the uptake of LDL at the single-cell level. In addition, multiplexing with other markers, such as LDLR expression, is possible to gain more information from a...
Application: FC
Format: Homog.
469.00€
Review
Phagocytosis Assay Kit (IgG-DyLight(TM) 405)
Phagocytosis Assay Kit (IgG-DyLight(TM) 405)

Item number: Cay601480-1

Cayman's Phagocytosis Assay Kit (IgG-DyLight(TM) 405) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout...
Application: Cell based assays, FC, Fluorescence
Format: Bead Based
369.00€
Review
Phagocytosis Assay Kit (IgG-DyLight(TM) 633)
Phagocytosis Assay Kit (IgG-DyLight(TM) 633)

Item number: Cay601490-1

Cayman's Phagocytosis Assay Kit (IgG-DyLight(TM) 633) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout...
Application: Cell based assays, FC, IF
Format: Bead Based
369.00€
Review
ROS Detection Cell-Based Assay Kit (DCFDA)
ROS Detection Cell-Based Assay Kit (DCFDA)

Item number: Cay601520-96

2,7-Dichlorofluoroscin Diacetate (DCFDA or 2,7-dichlorodihydrofluoroscein diacetate) is a widely-used, cell-permeable redox sensitive fluorescent probe that is oxidized by ROS and certain RNS to yield the highly fluorescent product 2,7-dichlorofluoroscein. Cayman's ROS Detection Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS...
Application: Cell based assays, FC
Format: Homog.
207.00€
Review
COX Fluorescent Inhibitor Screening Assay Kit
COX Fluorescent Inhibitor Screening Assay Kit

Item number: Cay700100-192

Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both COX and peroxidase activities. The COX component converts arachidonic acid to a hydroperoxy endoperoxide (PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (PGH2), the precursor of PGs, thromboxanes, and prostacyclins. It is now...
Application: COX inhibitor screening
719.00€
Review
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