Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the... read more »
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Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

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Antioxidant Assay Kit
Antioxidant Assay Kit

Item number: Cay709001-96

Cayman's Antioxidant Assay can be used to measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. Aqueous- and lipid-soluble antioxidants are not separated in this protocol, thus the combined antioxidant activities of all its constituents including vitamins, proteins, lipids,...
Application: Antioxidant capacity determination
360.00€ *
Review
Nitrate/Nitrite Colorimetric Assay Kit
Nitrate/Nitrite Colorimetric Assay Kit

Item number: Cay780001-192

Cayman's Nitrate/Nitrite Colorimetric Assay Kit provides an accurate and convenient method for measurement of total and individual nitrate and nitrite concentrations in a simple two-step process utilizing nitrate and nitrite standards. The first step is the conversion of nitrate to nitrite utilizing nitrate...
Keywords: Nitric Oxide Metabolite Detection Kit
Application: Nitrate detection, Nitrite detection
291.00€ *
Review
Estradiol ELISA Kit
Estradiol ELISA Kit

Item number: Cay501890-96

Estradiol is a steroid hormone produced from testosterone via the aromatase system in the granulosa cells of ovarian follicles. It is instrumental in the development of secondary sex characteristics at puberty and in the menstrual cycle. Plasma levels of estradiol peak during the follicular phase of the menstrual...
Keywords: 17beta-Estradiol, 17beta-Oestradiol, E2, Estradiol EIA Kit, beta-Estradiol
Application: Competitive ELISA
From 311.00€ *
Review
Amplite(TM) Choline Quantitation Kit
Amplite(TM) Choline Quantitation Kit

Item number: ABD-40007

Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis). It is a major source for methyl groups via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways....
Application: Cholin quantification
279.00€ *
Review
Amplite(TM) Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
Amplite(TM) Colorimetric Alkaline Phosphatase Assay Kit...

Item number: ABD-11950

Alkaline phosphatase (ALP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. An important use of alkaline phosphatase is as a label for...
Keywords: pNPP, AP, ALP, EC=3.1.3.1
Application: Alkaline phosphatase quantification, HTS
208.00€ *
Review
Hemoglobin Colorimetric Assay Kit
Hemoglobin Colorimetric Assay Kit

Item number: Cay700540-192

Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Beyond binding and transporting oxygen, hemoglobin also binds CO2, carbon monoxide (CO), and nitric...
Keywords: Hb, Hgb
Application: Hemoglobin quantification
335.00€ *
Review
Amplite(TM) Colorimetric Acetylcholinesterase Assay Kit
Amplite(TM) Colorimetric Acetylcholinesterase Assay Kit

Item number: ABD-11400

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to...
Application: Acetylcholinesterase assays
279.00€ *
Review
NF-kappaB (p65) Transcription Factor Assay Kit
NF-kappaB (p65) Transcription Factor Assay Kit

Item number: Cay10007889-96

Cayman's NF-kappaB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility...
Keywords: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide...
Application: NF-kappaB binding detection
Species reactivity: human, mouse, rat
661.00€ *
Review
COX-2 (human) Inhibitor Screening Assay Kit
COX-2 (human) Inhibitor Screening Assay Kit

Item number: Cay701080-96

The COX-2 (human) Inhibitor Screening Assay directly measures PGF2alpha by stannous chloride (SnCl2) reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using an antiserum specific to PGF2alpha. This assay includes recombinant human COX-2 and is an excellent...
Keywords: Cyclooxygenase 2
Application: Inhibitor screening
584.00€ *
Review
Amplite(TM) Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Amplite(TM) Fluorimetric Lysyl Oxidase Assay Kit *Red...

Item number: ABD-15255

This kit offers a sensitive fluorescent assay for lysyl oxidase activity that utilizes 1,5-diaminopentane as substrate, and released hydrogen peroxide is detected using our Amplite HRP substrate in HRP-coupled reactions.
Keywords: EC=1.4.3.13
Application: Lysyl oxidase activity assay
418.00€ *
Review
COX Fluorescent Inhibitor Screening Assay Kit
COX Fluorescent Inhibitor Screening Assay Kit

Item number: Cay700100-192

Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both COX and peroxidase activities. The COX component converts arachidonic acid to a hydroperoxy endoperoxide (PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (PGH2), the...
Keywords: Cyclooxygenase Fluorescent Inhibitor Screening Assay Kit
Application: COX inhibitor screening
895.00€ *
Review
Amplite(TM) Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
Amplite(TM) Fluorimetric Proteasome 20S Activity Assay...

Item number: ABD-13456

Our Amplite(TM) Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activity. Cleavage of...
Keywords: LLVY-R110, chymotrypsin-like protease activity
Application: Proteasome 20S activity assay, HTS
418.00€ *
Review
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