iFluor(TM) 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*

Ex: 541 nm. Em: 557 nm. Soluble in: Water. iFluorT 546 is a bright orange fluorescent dye. iFluorT 546-labeled anti-IgG conjugates exhibit bright fluorescence signal and good photostability. Used for stable signal generation in imaging and flow cytometry, the fluorescence intensity of iFluorT 546 conjugates is pH-insensitive from pH 4 to pH 11. The iFluorT 546-labeled antibody conjugates can be well excited with either Nd:YAG laser (~532 nm) or Helium-Neon laser (~543 nm). iFluorT 546 family has the spectral properties essentially identical to those of Alexa Fluor® 546. Under the same conditions we tested, iFluorT 546 antibody conjugates are brighter and more photostable than the corresponding Alexa Fluo® 546. These spectral and labeling characteristics make the iFluorT 546 dye family a superior alternative to Alexa Fluor® 546. In addition, iFluorT 546 secondary antibody conjugates give higher signal/background ratios than the corresponding Alexa Fluor® 546-labeled conjugates.