Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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CF(R)594 Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)594 Donkey Anti-Chicken IgY (IgG) (H+L), Highly...

Item number: BOT-20167

This product is prepared by labeling highly cross-adsorbed donkey anti-chicken IgY (H+L) with CF(R)594 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF(R)594 dye...
Keywords: CF(R)594 Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: chicken
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CF(R)633 Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)633 Donkey Anti-Chicken IgY (IgG) (H+L), Highly...

Item number: BOT-20168

This product is prepared by labeling highly cross-adsorbed donkey anti-chicken IgY (H+L) with CF(R)633 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF(R)633 dye...
Keywords: CF(R)633 Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: chicken
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CF(R)488A Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)488A Donkey Anti-Guinea Pig IgG (H+L), Highly...

Item number: BOT-20169

This product is prepared by labeling highly cross-adsorbed donkey anti-guinea pig IgG (H+L) with CF(R)488A dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF(R)488A...
Keywords: CF(R)488A Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: guinea pig
From 143.00€ *
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CF(R)594 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)594 Donkey Anti-Guinea Pig IgG (H+L), Highly...

Item number: BOT-20170

This product is prepared by labeling highly cross-adsorbed donkey anti-guinea pig IgG (H+L) with CF(R)594 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF(R)594...
Keywords: CF(R)594 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: guinea pig
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CF(R)633 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)633 Donkey Anti-Guinea Pig IgG (H+L), Highly...

Item number: BOT-20171

This product is prepared by labeling highly cross-adsorbed donkey anti-guinea pig IgG (H+L) with CF(R)633 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF(R)633...
Keywords: CF(R)633 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: guinea pig
From 143.00€ *
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CF(R)488A Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)488A Rabbit Anti-Sheep IgG (H+L), Highly...

Item number: BOT-20172

This product is prepared by labeling highly cross-adsorbed rabbit anti-sheep IgG (H+L) with CF(R)488A dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF(R)488A dye has...
Keywords: CF(R)488A Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Rabbit
Species reactivity: sheep
From 143.00€ *
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CF(R)594 Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)594 Rabbit Anti-Sheep IgG (H+L), Highly...

Item number: BOT-20173

This product is prepared by labeling highly cross-adsorbed rabbit anti-sheep IgG (H+L) with CF(R)594 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF(R)594 dye...
Keywords: CF(R)594 Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Rabbit
Species reactivity: sheep
From 143.00€ *
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CF(R)633 Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)633 Rabbit Anti-Sheep IgG (H+L), Highly...

Item number: BOT-20174

This product is prepared by labeling highly cross-adsorbed rabbit anti-sheep IgG (H+L) with CF(R)633 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF(R)633 dye has...
Keywords: CF(R)633 Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Rabbit
Species reactivity: sheep
From 143.00€ *
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CF(R)640R Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)640R Goat Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20175

This product is prepared by labeling highly cross-adsorbed goat anti-mouse IgG (H+L) with CF(R)640R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF(R)640R dye has...
Keywords: CF(R)640R Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Goat
Species reactivity: mouse
From 143.00€ *
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CF(R)640R Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)640R Goat Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20176

This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF(R)640R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF(R)640R dye...
Keywords: CF(R)640R Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)640R Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)640R Donkey Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20177

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF(R)640R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF(R)640R dye...
Keywords: CF(R)640R Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: mouse
From 143.00€ *
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CF(R)640R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)640R Donkey Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20178

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF(R)640R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF(R)640R dye...
Keywords: CF(R)640R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: rabbit
From 143.00€ *
Review
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