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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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Anti-Pig IgG-heavy and light chain cross-adsorbed, HRP conjugated
Anti-Pig IgG-heavy and light chain cross-adsorbed, HRP...

Item number: A100-205P

Bethyl
Format - Whole IgG, minimum reactivity to bovine, chicken, horse, human, mouse, rabbit and rat
Application: WB, IHC, ICC, ELISA
Host: Goat
Species reactivity: pig
167.00€ *
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Anti-Bird IgG-heavy and light chain, FITC conjugated
Anti-Bird IgG-heavy and light chain, FITC conjugated

Item number: A140-110F

Bethyl
Format - Whole IgG
Application: IHC, ICC, FC, IF
Host: Goat
Species reactivity: bird
183.00€ *
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Anti-Human IgG-Fc Fragment
Anti-Human IgG-Fc Fragment

Item number: A80-104A

Bethyl
Format - Whole IgG
Application: WB, IHC, ICC, ELISA
Host: Goat
Species reactivity: human
144.00€ *
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Anti-Human IgE
Anti-Human IgE

Item number: A80-108A

Bethyl
Format - Whole IgG
Application: WB, IHC, ICC, ELISA
Host: Goat
Species reactivity: human
238.00€ *
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Anti-Human IgE, Biotin conjugated
Anti-Human IgE, Biotin conjugated

Item number: A80-108B

Bethyl
Format - Whole IgG
Application: WB, IHC, ICC, ELISA
Host: Goat
Species reactivity: human
285.00€ *
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Anti-Human IgE
Anti-Human IgE

Item number: A80-109A

Bethyl
Format - Whole IgG
Application: WB, IHC, ICC, ELISA
Host: Rabbit
Species reactivity: human
238.00€ *
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Anti-Human IgM (Mu Chain) [Goat] Peroxidase conjugated
Anti-Human IgM (Mu Chain) [Goat] Peroxidase conjugated

Item number: 609-1307

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: human
272.00€ *
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CF(R)350 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)350 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2...

Item number: BOT-20145

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)350 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue...
Keywords: CF(R)350 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Host: Goat
Species reactivity: mouse
From 143.00€ *
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CF(R)350 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)350 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20146

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)350 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue...
Keywords: CF(R)350 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)350 Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)350 Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed,...

Item number: BOT-20147

Biotium
This product is prepared by labeling highly cross-adsorbed goat anti-rat IgG (H+L) with CF(R)350 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue fluorescent CF(R)350...
Keywords: CF(R)350 Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Goat
Species reactivity: rat
From 143.00€ *
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CF(R)350 Donkey Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)350 Donkey Anti-Sheep IgG (H+L), Highly...

Item number: BOT-20148

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-sheep IgG (H+L) with CF(R)350 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue fluorescent...
Keywords: CF(R)350 Donkey Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Donkey
Species reactivity: sheep
From 143.00€ *
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CF(R)350 Rabbit Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)350 Rabbit Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20149

Biotium
This product is prepared by labeling high quality rabbit anti-mouse IgG (H+L) with CF(R)350 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue fluorescent CF(R)350 dye...
Keywords: CF(R)350 Rabbit Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Host: Rabbit
Species reactivity: mouse
From 143.00€ *
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2 from 649 pages
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