Anti-phospho-S6 Ribosomal Protein(Ser235/236)

Anti-phospho-S6 Ribosomal Protein(Ser235/236)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
R2031-29C.20 20 µl - -

4 - 22 business days

357.00€
R2031-29C.100 100 µl - -

4 - 22 business days

650.00€
 
To effectively promote growth and cell division in a sustained manner, growth factors and... more
Product information "Anti-phospho-S6 Ribosomal Protein(Ser235/236)"
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunohistochemistry (paraffin/frozen): 1:400, Immunofluorescence (IF-IC): 1:100, Flow Cytometry: 1:25, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: R2031-29C

Properties

Application: FC, IF, IHC, WB
Antibody Type: Monoclonal
Clone: 8A30(D57.2.2E)
Host: Rabbit
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

Database Information

Handling & Safety

Storage: -20°C
Shipping: °C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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