Anti-phospho-MAP Kinase p44/42, (Erk1/2) (Thr202/Tyr204) (MAPK, Erk1/2, Extracellular Signal Regulat

Anti-phospho-MAP Kinase p44/42, (Erk1/2) (Thr202/Tyr204) (MAPK, Erk1/2, Extracellular Signal Regulat
Item number Size Datasheet Manual SDS Delivery time Quantity Price
M2352-20E.200 200 µl - -

3 - 19 business days*

559.00€
 
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine... more
Product information "Anti-phospho-MAP Kinase p44/42, (Erk1/2) (Thr202/Tyr204) (MAPK, Erk1/2, Extracellular Signal Regulat"
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 , Immunoprecipitation: 1:50 , Immunohistochemistry (Paraffin): 1:100 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: M2352-20E

Properties

Application: IHC, IP, WB
Antibody Type: Monoclonal
Clone: 9B154(20G11)
Conjugate: No
Host: Rabbit
Reactivity: Drosophila, Human, Monkey, Mouse, Swine, Rat, Zebrafish
Immunogen: Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
Purity: Supernatant
Format: Supernatant

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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