Anti-phospho-Bad,

Anti-phospho-Bad,
Item number Size Datasheet Manual SDS Delivery time Quantity Price
B0003-51C2.1 1 kit - -

3 - 19 business days*

708.00€
 
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from... more
Product information "Anti-phospho-Bad,"
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from bind- ing to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 protein to prevent an association between Bad and Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10)., , Applications: , Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: B0003-51C2

Properties

Application: WB
Antibody Type: Monoclonal
Clone: 9j107
Conjugate: No
Host: Mouse
Immunogen: Synthetic phosphopeptides (KLH-coupled) corresponding to sites surrounding specific phosphorylated residues.
Purity: Purified by Immunoaffinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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