Anti-phospho-4E Binding Protein 1, (Thr37/46) (Protein Synthesis Initiation Factor 4E Binding

Anti-phospho-4E Binding Protein 1, (Thr37/46) (Protein Synthesis Initiation Factor 4E Binding
Item number Size Datasheet Manual SDS Delivery time Quantity Price
0004-18J.100 100 µl - -

3 - 19 business days*

564.00€
 
When bound to eIF4E, 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation. Upon... more
Product information "Anti-phospho-4E Binding Protein 1, (Thr37/46) (Protein Synthesis Initiation Factor 4E Binding"
When bound to eIF4E, 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4), while phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry, and Flow cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. For Western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Immunohistochemistry (Paraffin): 1:1600 (Citrate/TBST), Immunofluorescence (IC): 1:200 , Flow Cytometry: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-phospho-4E-BP1, Anti-phospho-PHAS-I, Anti-phospho-Eif4ebp1, Anti-phospho-eIF4E-binding protein 1, Anti-phospho-Eukaryotic translation initiation factor 4E-binding protein 1, Anti-phospho-Phosphorylated heat- and acid-stable protein regulated by insul
Supplier: United States Biological
Supplier-Nr: 0004-18J

Properties

Application: FC, ICC, IF, IHC, WB
Antibody Type: Monoclonal
Clone: 9B142(236B4)
Conjugate: No
Host: Rabbit
Reactivity: Drosophila, Human, Monkey, Mouse, Rat
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 (KLH-coupled)
Purity: Ascites
Format: Ascites

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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