Anti-Histone H3K36Me2

Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various posttranslational modifications, including mono-, di-, and trimethylation at histone H3 lysine 36 (H3K36). Dimethylation of H3K36 is catalyzed by SET domain-containing histone methyltransferases including SET2, HYPB, NSD1, and ASH1L, and mutation of these methyltransferases is associated with various diseases, including multiple myeloma and Sotos syndrome. H3K36Me2 is enriched at the coding regions of genes and correlates with the initiation, but not maintenance, of active transcription, heterochromatin maintenance, and DNA break repair. Cayman's Histone H3K36Me2 Polyclonal Antibody can be used for ELISA and Western blot applications. It detects H3K36Me2 at ~16 kDa from human samples.Synonyms: Histone H3 containing dimethylated lysine 36. Immunogen: Synthetic peptide from human histone H3 amino acids 26-44 (dimethyl K36) conjugated to KLH. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Rabbit. Applications: ELISA and WB. Cross Reactivity: (+) Histone H3 amino acids 26-44 (~10%), (+) Histone H3 dimethylated at lysine 9 (~10%), (+) Histone H3 monomethylated at lysine 36 (~10%), (+) Histone H3 trimethylated at lysine 36 (~10%). UniProt Accession #: P68431.