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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
U1000-07D.100 | 100 µl | - | - | 3 - 19 business days* | 514.00€ |
You can also order using info@biomol.com or FAX at 0049-40-85326022
Bigger quantity required? Request Bulk
Bigger quantity required? Request Bulk
Ubiquitin is a conserved polypeptide unit that plays an important role in the... more
Product information "Anti-Ubiquitin"
Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1, the activated ubiquitin is subsequently transfered to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IkB, p53, cdc25a and bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response and apoptosis (4-7). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunohistochemistry (Paraffin): 1:1600. Antigen retrieval by heating in citric acid buffer is recommended., Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | Anti-UBB, Anti-Polyubiquitin-B |
Supplier: | United States Biological |
Supplier-Nr: | U1000-07D |
Properties
Application: | IHC, WB |
Antibody Type: | Polyclonal |
Conjugate: | No |
Host: | Rabbit |
Reactivity: | Human, Mouse, Rat |
Immunogen: | Synthetic peptide corresponding to the amino terminus of the human ubiquitin protein (KLH). |
Purity: | Purified by Protein A affinity chromatography. |
Format: | Affinity Purified |
Database Information
KEGG ID : | K02927 | Find alternatives |
UniProt ID : | P62988 | Find alternatives |
Gene ID | GeneID 7311 | Find alternatives |
Handling & Safety
Storage: | -20°C (-20°C Glycerol) |
Shipping: | +4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
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