Product information "Anti-Transforming Growth Factor beta Induced (BIGH3, TGFBI, ig-h3)"
Beta-IG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683aa secretory protein induced by TGF-b that plays a role in cell adhesion, differentiation, and apoptosis (1-4). Beta-IG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxyl terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM Beta-IG-H3 is found in a wide variety of tissues (8-12). Mutations in the Beta-IG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (IF-IC): 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.