Anti-Tetanus Toxin, Clostridium Tetani (Tetanospasmin, TeNT)

Anti-Tetanus Toxin, Clostridium Tetani (Tetanospasmin, TeNT)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
T2962-01.1 1 mg - -

3 - 19 business days

Tetanus toxin is polypeptide composed of two chains [A chain (55kD) and B chain (105kD)]. The... more
Product information "Anti-Tetanus Toxin, Clostridium Tetani (Tetanospasmin, TeNT)"
Tetanus toxin is polypeptide composed of two chains [A chain (55kD) and B chain (105kD)]. The toxin is carried to CNS via bloodstream. Tetanus toxin binds at neuromuscular synapses via ganglioside receptors (sialic acid) via B-chain. The toxin is then internalized via receptor-mediated endocytosis. The A chain is thought to be the toxic subunit by analogy to diptheria and cholera toxin. It has no known effect on any cellular enzyme systems. The action of the holotoxin (A + B chains) is to interfere with synaptic transmission by preferentially inhibiting the release of inhibitory neurotransmitters such as glycine and GABA from inhibitory neurons. This inhibition causes rigid muscle contraction-spastic paralysis. Tetanus toxin is an exotoxin. These toxins are secreted by a bacterial cell into surrounding fluids. Exotoxins are produced by both Gram-negative and Gram-positive bacteria. Most exotoxins are readily destroyed by heat. They can be converted into toxoids that are used as vaccines. Exotoxins are extrememly powerful biological poisons as in the case of tetanus. It has been estimated that 1mg of purified tetanus toxin could kill millions of mice. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: T2962-01


Application: ELISA, Neutr., WB
Antibody Type: Monoclonal
Clone: 9B138
Conjugate: No
Host: Mouse
Immunogen: Supernatant from C. tetani.
Purity: Purified by Protein G chromatography. >95% pure.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Our products are for laboratory research use only: Not for administration to humans!
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