Anti-SIRP, alpha1, aa487-503, (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a) (FITC )

Anti-SIRP, alpha1, aa487-503, (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a) (FITC )
Item number Size Datasheet Manual SDS Delivery time Quantity Price
S1013-87H1.100 100 µg - -

3 - 19 business days*

542.00€
 
CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein... more
Product information "Anti-SIRP, alpha1, aa487-503, (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a) (FITC )"
CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), , macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of , immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 1:10. Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul., Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line , Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-Bit, Anti-Sirp-alpha-2, Anti-Sirp-alpha-3, Anti-Sirp-alpha-1, Anti-MyD-1 antigen, Anti-SHP substrate 1, Anti-Macrophage fusion receptor, Anti-Inhibitory receptor SHPS-1, Anti-Signal-regulatory protein alpha-1, Anti-Signal-regulatory protein alpha-2
Supplier: United States Biological
Supplier-Nr: S1013-87H1

Properties

Application: FC
Antibody Type: Monoclonal
Clone: 10B870
Conjugate: FITC
Host: Mouse
Reactivity: Human
Immunogen: Monocyte-derived dendritic cells.
Purity: Purified by Protein G affinity chromatography.
Format: Affinity Purified

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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