Anti-SFRP4 (Secreted Frizzled Related Protein-4, DDC-4, FrpAP, frpHE, FrzB-2)

sFRP4 belongs to the sFRP family whose members contain a cysteine-rich domain homologous to the Wnt-binding domain of the Frizzled proteins. SFRP4 is a circulating protein that can function as a phosphatonin as well as an antagonist of Wnt signaling. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml. Detection limit for rhsFRP-4 is ~2ng/well., Western Blot: 0.1-0.2ug/ml. Detection limit for rhsFRP-4 is ~20ng/lane under non-reducing and reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.