Anti-SFPQ, CT (SFPQ, PSF, Splicing factor, proline- and glutamine-rich, 100kD DNA-pairing protein, D

Anti-SFPQ, CT (SFPQ, PSF, Splicing factor, proline- and glutamine-rich, 100kD DNA-pairing protein, D
Item number Size Datasheet Manual SDS Delivery time Quantity Price
041622.200 200 µl - -

1 - 19 business days

557.00€
 
DNA-and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing... more
Product information "Anti-SFPQ, CT (SFPQ, PSF, Splicing factor, proline- and glutamine-rich, 100kD DNA-pairing protein, D"
DNA-and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing, in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1, in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends, in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Applications: Suitable for use in Western Blot, ELISA, Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-500, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-PSF, Anti-SFPQ, Anti-hPOMp100, Anti-100 kDa DNA-pairing protein, Anti-PTB-associated-splicing factor, Anti-Splicing factor, proline- and glutamine-rich, Anti-DNA-binding p52/p100 complex, 100 kDa subunit
Supplier: United States Biological
Supplier-Nr: 041622

Properties

Application: ELISA, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human
Immunogen: SFPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 675-705 amino acids from the C-terminal region of human SFPQ.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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