Anti-Separase (Separin, Caspase-like Protein ESPL1, ESP1, Extra Spindle Poles-like 1, Extra Spindle

Anti-Separase (Separin, Caspase-like Protein ESPL1, ESP1, Extra Spindle Poles-like 1, Extra Spindle
Item number Size Datasheet Manual SDS Delivery time Quantity Price
S0952-01.100 100 µl - -

3 - 19 business days

623.00€
 
In normal cellular proliferation, the maintenance of constant centriole numbers is strongly... more
Product information "Anti-Separase (Separin, Caspase-like Protein ESPL1, ESP1, Extra Spindle Poles-like 1, Extra Spindle"
In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis. Applications: Suitable for use in Peptide ELISA. Other applications not, tested. Recommended Dilution: Peptide ELISA: 1:100-1:2000, Optimal dilutions to be determined by the researcher. Cellular Localization: Cytoplasmic and Nuclear. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: S0952-01

Properties

Application: ICC, IHC, IP, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human
Immunogen: A synthetic peptide made to an N-terminal region (within residues 1-100) of the human separase protein. [Swiss-Prot# Q14674]. Homology: 100% with human, 88% with cow, and 76% with mouse protein.
Purity: Purified by peptide immunoaffinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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