Anti-RORC (Nuclear Receptor ROR-gamma, Nuclear Receptor RZR-gamma, Nuclear Receptor Subfamily 1 Grou

Retinoic acid-related Orphan Receptor gamma (RORg, TOR, RORC, NR1F3) is a member of the orphan nuclear receptor family. RORg is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. RORg plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of RORg, RORgt, has been shown to be specifically expressed in the thymus. By direct FLISA, R1183-30G has been shown to specifically recognize the RORyt isoform, and not RORC. Applications: Suitable for use in Direct FLISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 cells, Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with RORyt peptide. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.