Anti-ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)

Anti-ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
R2998-02D.20 20 µl - -

1 - 19 business days

330.00€
R2998-02D.100 100 µl - -

1 - 19 business days

566.00€
 
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream... more
Product information "Anti-ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)"
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of GTPase Rho and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified (ROCK1 and ROCK2). ROCK is composed of N-terminal catalytic, coiled-coil and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis and smooth muscle contraction (4-6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: R2998-02D

Properties

Application: IP, WB
Antibody Type: Monoclonal
Clone: 8A34(C8F7)
Conjugate: No
Host: Rabbit
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic peptide (KLH-coupled) corresponding to the central sequence of human ROCK1.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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