Anti-RNA Polymerase II (Rpb1 subunit, CTD) 220kD

Anti-RNA Polymerase II (Rpb1 subunit, CTD) 220kD
Item number Size Datasheet Manual SDS Delivery time Quantity Price
R2039-22N.100 100 µl - -

1 - 19 business days

496.00€
 
RNA polymerase II is a complex of 12 proteins that participates in transcription, mRNA... more
Product information "Anti-RNA Polymerase II (Rpb1 subunit, CTD) 220kD"
RNA polymerase II is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair (1,2). The largest subunit, Rpb1, contains a unique heptapeptide: Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7 , which is repeated up to 52 times in the carboxy-terminal domain (CTD) of Rpb1 (3). This CTD is phosphorylated by cyclin-dependent kinases (cdks), p44/42 MAPK, DNA-PKcs, and c-Abl (4). DNA damage caused by UV-light, hydrogen peroxide, or cisplatin results in ubiquitination and proteasomal degradation of Rpb1 (1,3). The kinase inhibitor 5,6-dichloro-1-b-Dribofuranosylbenzimidazole prevents ubiquitination of Rpb1, suggesting that CTD phosphorylation is required for proteolysis (5). Applications: Suitable for use in ChIP, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membranes with diluted antibody, 1X TBS, 5% w/v nonfat dry milk, 0.1% Tween-20 at 4ºC with gentle shaking overnight., Immunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: R2039-22N

Properties

Application: IP, WB
Antibody Type: Monoclonal
Clone: 8A33(4H8)
Conjugate: No
Host: Mouse
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic phosphopeptide (KLH-coupled) containing 10 heptapeptide repeats [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] in which Ser5 is phosphorylated.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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