Anti-Rad50 (DNA Repair Protein RAD50, hRad50, hsRAD50, Mrell, Nbs1 Complex, RAD50-2, RAD50 Homolog (

Anti-Rad50 (DNA Repair Protein RAD50, hRad50, hsRAD50, Mrell, Nbs1 Complex, RAD50-2, RAD50 Homolog (
Item number Size Datasheet Manual SDS Delivery time Quantity Price
R0450-01J.100 100 µl - -

3 - 19 business days*

498.00€
 
RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an... more
Product information "Anti-Rad50 (DNA Repair Protein RAD50, hRad50, hsRAD50, Mrell, Nbs1 Complex, RAD50-2, RAD50 Homolog ("
RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 1-5ul, Western Blot: 1:1000-1:10,000 detects a band at ~153kD, Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell extract, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: R0450-01J

Properties

Application: IP, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human
Immunogen: Human Rad50 zinc hook region (amino acids 628-787) expressed in E. coli.
Purity: Serum
Format: Serum

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow. more
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Anti-Rad50 (DNA Repair Protein RAD50, hRad50, hsRAD50, Mrell, Nbs1 Complex, RAD50-2, RAD50 Homolog ("
Write a review
or to review a product.
Viewed